PUBLICATIONS

2024

126. Zhang, C., Subthain, H., Guo, F., Fang, P., Zheng, S., Shen, M., Jiang, X., Gao, Z., Meng, C. *, Li, S.*, Du, L. * Terminal deoxynucleotidyl transferase: Properties and applications. Eng. Microbiol. 2025, 5, 100179. | PDF




125. Zheng, S. #, Gao, Z. #, Jiang, Y., Guo, J., Cheng, F., Wang, X., Yu, H., Hu, B., Meng, C.*, Li, S.*, Zhang, X.* Light-driven enzymatic conversion of mevastatin into pravastatin by coupling P450sca-2 with cyanobacterial photosynthetic system. Green Synth. Catal. 2024, doi.org/10.1016/j.gresc.2024.07 .001. | PDF




124. Zhang, G., Du, J., Zhang, C., Zhao, Z., Chen, Y., Liu, M., Chen, J., Fan, G., Ma, L.*, Li, S.*, Liu, K.* Identification of a PET hydrolytic enzyme from the human gut microbiome unveils potential plastic biodegradation in human digestive tract. Int. J. Biol. Macromol. 2024, 283, 137732.| PDF



123. Wang, A., Wang, Y., You, Y., Huang, Z., Zhang, X., Li, S. *, Chen, H. * One-pot Biocatalytic Conversion of Chemically Inert Hydrocarbons into Chiral Amino Acids through Internal Cofactor and H2O2 Recycling. Angew. Chem. Intl. Ed. 2024, 63, 48, e202410260. | PDF




122. Chen, J. #, Jia, Y. #, Sun, Y. #*, Liu, K. #, Zhou, C., Liu, C., Li, D., Liu, G., Zhang, C., Yang, T., Huang, L., Zhuang, Y., Wang, D., Xu, D., Zhong, Q., Guo, Y., Li, A., Seim, I., Jiang, L., Wang, L., Lee, M. Y. S., Liu, Y., Wang, D., Zhang, G., Liu, S., Wei, X., Yue, Z., Zheng, S., Shen, X., Wang, S., Qi, C., Chen, J., Ye, C., Zhao, F., Wang, J., Fan, J., Li, B., Sun, J., Jia, X., Xia, Z., Zhang, H., Liu, J., Zheng, Y., Liu, X., Wang, J., Yang, H., Kristiansen, K., Xu, X., Mock, T. *, Li, S. *, Zhang, W. *, Fan, G. * Global marine microbial diversity and its potential in bioprospecting. Nature 2024, 633, 371-379. | PDF



121. Tang, D., Zheng, X., Zhao, Y., Zhang, C., Chen, C., Chen, Y., Du, L. *, Liu, K. *, Li, S. * Engineered Microbial Consortium for De Novo Production of Sclareolide. J. Agric. Food Chem. 2024, 72, 36, 19977-19984. | PDF



120. You, C. #, Pan, Y. #, Liu, R., Li, S. *, Feng, Y. * Structural basis for substrate flexibility of the O-methyl transferase MpaG' involved in mycophenolic acid biosynthesis. Protein Sci. 2024, 33(9), e5144. | PDF



119. Jiang, Y. #, Li, P. #, Li, Z., Zhang, B. *, Li, S. * Photoenzymatic synthesis of 1-alkenes and hydroxyl fatty acids by cascading a COF photocatalyst and P450 peroxygenases. Sci. Bull. 2024,69(21), 3350-3354.| PDF



118. Zhou, T., Feng, J., Wang, Y., Li, S.*, Wang, B.* Substrate Conformational Switch Enables the Stereoselective Dimerization in P450 NascB: Insights from Molecular Dynamics Simulations and Quantum Mechanical/Molecular Mechanical Calculations. J. Am. Chem. Soc. Au 2024, 4, 1591-1604.| PDF



117. Zhang, X., Cheng, F., Guo, J., Zheng, S., Wang, X., Li, S. * Enzymatic synthesis of organoselenium compounds via C‒Se bond formation mediated by sulfur carrier proteins. Nat. Synth. 2024, 3, 477-487. | PDF



116. Liu, M., Li, S. * Nitrile biosynthesis in nature: how and why? Nat. Prod. Rep. 2024, 41, 649-671. | PDF



115. Zhang, C., Zhang, G., Chen, Y., Zheng, S., Du, J., Zhao, Z., Zhao, Y., Wang, N., Chen, C., Gao, Z. *, Li, S. *, Liu, K. * Sphingobacterium tenebrionis sp. nov., isolated from intestine of mealworm. Int. J. Syst. Evol. Microbiol. 2024, 74, 006455. | PDF



114. Qiu. Z. #, Liu, X. #, Yu, J., Zhao, Y., Zhao, G., Li, S., Liu, K. *, Du, L. *, Ma, L. * Efficient conversion of aromatic and phenylpropanoid alcohols to acids by the cascade biocatalysis of alcohol and aldehyde dehydrogenases. Synth. Syst. Biotechnol. 2024, 9(2), 187-195. | PDF



113. Li, S., Li, Z., Zhang, G., Urlacher. B. V., Ma, L. *, Li, S. * Functional analysis of the whole CYPome and Fdxome of Streptomyces venezuelae ATCC 15439. Eng. Microbiol. 2024, 4, 100166. | PDF



112. Sui, X., Wang, J., Zhao, Z., Liu, B., Liu, M., Liu, M., Shi, C., Feng, X., Fu, Y., Shi, D., Li, S., Qi, Q., Mian. M., Zhao, G. * Phenolic compounds induce ferroptosis-like death by promoting hydroxyl radical generation in the Fenton reaction. Commun. Biol. 2024, 7,199. | PDF

111. Zhu, L., Yang, Q., Wang, D., Niu, L., Pan, Z., Li, S., Li, Y., Zhang, W., Wu, C. Deciphering the Biosynthesis and Physiological Function of 5‑Methylated Pyrazinones Produced by Myxobacteria. ACS Cent. Sci. 2024, 10, 555-568. | PDF



2023

110. Xia, Y. #, Zhu, G. #, Zhang, X. #, Li, S., Du, L.*, Zhu, W.* Biosynthesis of 4‑Acyl-5-aminoimidazole Alkaloids Featuring a New Friedel−Crafts Acyltransferase. J. Am. Chem. Soc. 2023, 145: 26308-26317. | PDF



109. Liu, K., Gao, W., Xu, W., Liu, H.* Deep-sea microorganisms acquired during Jiaolong expedition. The Innovation Life 2023, 1(2): 100029. | PDF



108. Li, Z. #, Zhang, L. #, Kang, X. #, Jiang, Y., Du, J., Zhang, X., Meng, L., Wu, Q., Du, L., Li, X., Hu, Y., Xie, Z., Jiang, X., Tang, Y., Wu, R. *, Guo, R. *, Li, S. * Molecular insights into the catalytic promiscuity of a bacterial diterpene synthase. Nat. Commun. 2023, 14: 4001. | PDF



107. Ma, L., Sun, T., Liu, Y., Zhao, Y., Liu, X., Li, Y., Chen, X., Cao, L., Kang, Q., Guo, J., Du, L., Wang, W. *, Li, S. * Enzymatic synthesis of indigo derivatives by tuning P450 BM3 peroxygenases. Synth. Syst. Biotechnol. 2023, 8: 452-461. | PDF



106. Pan, Y.#, Li, G.#, Liu, R., Guo, J., Liu, Y., Liu, M., Zhang, X., Chi, L., Xu, K., Wu, R., Zhang, Y., Li, Y., Gao, X.*, Li, S.* Unnatural activities and mechanistic insights of cytochrome P450 PikC gained from site-specific mutagenesis by non-canonical amino acids. Nat. Commun. 2023, 14:1669. | PDF



105. Liu, R., Pan, Y., Wang, N., Tang, D., Urlacher, B. V., Li, S.* Comparative biochemical characterization of mammalian-derived CYP11A1s with cholesterol side-chain cleavage activities. J. Steroid Biochem. 2023, 229, 106268. | PDF



104. Yang, F.#, Sang, M.#, Liu, J.-R., Zhao, H.-M.,Zou, Y., Wu, W., Yu, Y., Ma, W., Zhang, Y., Zhang, W.*, Lin, H.-W.* Somalactams  A–D:  Anti-inflammatory  Macrolide Lactams with Unique Ring Systems from an Arctic Actinomycete Strain. Angew. Chem. Intl. Ed.  2023, 62, e202218085. | PDF



103. Chi, X. *, Zhao, Z., Han, Q., Yan, H., Ji, B., Chai, Y., Li, S., Liu, K. * Insights into autotrophic carbon fixation strategies through metagonomics in the sediments of seagrass beds. Mar. Environ. Res. 2023, 188, 106002. | PDF



102. Liu, K., Xu, Z., Zhao, Z., Chen, Y., Chai, Y., Ma, L., Li, S.* A dual fluorescence assay enables high-throughput screening for PET hydrolases. ChemSusChem 2023, 16, e2022020. | PDF





2022

101. Li, S. #, Chi, L. #, Li, Z., Liu, M., Liu, R., Sang, M., Zheng, X., Du, L., Zhang, W., Li, S.* Discovery of venediols by activation of a silent type I polyketide biosynthetic gene cluster in Streptomyces venezuelae ATCC 15439. Tetrahedron 2022, 133072. |  PDF



100. Guo, F. #, Zhang, X. #, You, C., Zhang, C., Li, F., Li, N., Xia, Y., Liu, M., Qiu, Z., Zheng, X., Ma, L., Zhang, G., Luo, L., Cao, F., Feng, Y., Zhao, G., Zhang, W., Li S., Du, L.* Diversifcation of phenolic glucosides by two UDP-glucosyltransferases featuring complementary regioselectivity. Micro. Cell.  Fact. 2022, 21, 208.   |  PDF


 

99. Liu, X.#, Li, F.#, Sun, T., Guo, J., Zhang, X., Zheng, X., Du, L., Zhang, W., Ma, L. *, Li, S. Three pairs of surrogate redox partners comparison for Class I cytochrome P450 enzyme activity reconstitution. Commun. Biol 2022, 5(1), 791. |  PDF
 


98. Zheng, S. #, Guo, J. #, Cheng, F., Gao, Z., Du, L., Meng, C.*, Li, S.*, Zhang, X.* Cytochrome P450s in algae: Bioactive natural product biosynthesis and light-driven bioproduction. Acta Pharm. Sin. B 2022,12(6), 2832-2844. |  PDF
 


97. Durairaj, P., Li, S.* Functional expression and regulation of eukaryotic cytochrome P450 enzymes in surrogate microbial cell factories.  Eng. Microbiol. 2022, 2(1), 100011.  |   PDF
 


96. Wang, Y.  #, Wang, P.  #, Cao, H., Ding, H., Su, H., Liu, S., Liu, G., Zhang, X., Li, C., Peng, M., Li, F., Li, S., Chen, Y., Chen, X. *, Zhang, Y. * Structure of Vibrio collagenase VhaC provides insight into the mechanism of bacterial collagenolysis.  Nat. Commun. 2022, 13(1): 566.  |   PDF


95. Gao, X. #, Su, Q. #, Li, J., Yang, W., Yao, B., Guo, J., Li, S., Liu C. * RNA-Seq analysis reveals the important co-expressed genes associated with polyphyllin biosynthesis during the developmental stages of Paris polyphylla. BMC Genom. 2022, 23, 559. |  PDF


94. Wu, M. #, Miao, L. #, Liu, Y. #, Qian, X., Hou, T., Ai, G., Yu, L., Ma, L., Gao, X., Qin, Y., Zhu, H., Du, L., Li, S., Tian, C., Li, D. *, Liu, Z. *, Liu, S. * Identification and characterization of a novel hydroxylamine oxidase, DnfA, that catalyzes the oxidation of hydroxylamine to N2. J. Biol. Chem. 2022, 298(9), 102372. |  PDF



2021

93. Guo, J., Li, F., Cheng, F., Ma, L., Liu, X., Durairaj, P., Zhang, G., Tang, D., Long, X., Zhang, W., Du, L., Zhang, X.*, Li, S. Bacterial biosynthetic P450 enzyme PikCD50N: A potential biocatalyst for the preparation of human drug metabolites.J. Org. Chem.2021,86, 14563-14571.|PDF

92. Jiang, Y. #, Peng, W. #, Li, Z. #, You, C., Zhao, Y., Tang, D., Wang, B. *, Li, S.*Unexpected reactions of α,β-unsaturated fatty acids provide insight into the mechanisms of CYP152 peroxygenases.Angew. Chem. Intl. Ed.2021, 60, 24694-24701.|PDF

91. Zhang, X., Xu, X., You, C., Yang, C., Guo, J., Sang, M., Geng, C., Cheng, F., Du, L., Shen, Y., Wang, S., Lan, H., Yang, F., Li, Y., Tang, Y., Zhang, Y., Bian, X.*, Li, S.*, Zhang, W.* Biosynthesis of chuangxinmycin featuring a deubiquitinase-like sulfurtransferase.Angew. Chem. Intl. Ed.2021, 133, 24623-24628.|PDF

90. Ma, L., Li, F., Zhang, X., Chen, H., Huang, Q., Su, J., Liu, X., Sun, T., Fang, B., Liu, K., Tang, D., Wu, D., Zhang, W., Du, L., Li, S. * Development of MEMS directed evolution strategy for multiplied throughput and convergent evolution of cytochrome P450 enzymes.Sci. China Life Sci.2021, 65(3), 550-560.|PDF

89. You, C., Li, F., Zhang, X., Ma, L., Zhang, Y., Zhang, W., and Li, S. * Structural basis for substrate speci?city of the peroxisomal acyl-CoA hydrolase MpaH’involved in mycophenolic acid biosynthesis.FEBS J.2021, 288, 5768-5780.|PDF

88. Dong, S. #, Chen, J. #, Zhang, X., Guo, F., Ma, L., You, C., Wang, X., Zhang, W., Wan, X., Liu, S., Yao, L., Li, S., Du, L. * and Feng, Y. * Structural basis for selective oxidation of phosphorylated ethylphenols by cytochrome P450 monooxygenase CreJ.Appl. Environ. Microbiol.2021, 87(11), e00018-21.|PDF

87. Zhang, X., Guo, J., Cheng, F., and Li, S. * Cytochrome P450 enzymes in fungal natural product biosynthesis.Nat. Prod. Rep.2021, 38, 1072-1099.|PDF

86. Du, L., and Li, S. * Compartmentalized biosynthesis of fungal natural products.Curr. Opin. Biotechnol.2021, 69, 128-135.|PDF

85. Li, Z., Li, S., Du, L., Zhang, X., Jiang, Y., Liu, W., Zhang, W., and Li, S. * Engineering Bafilomycin High-Producers by Manipulating Regulatory and Biosynthetic Genes in the Marine BacteriumStreptomyces lohii.Mar. Drugs, 2021, 19, 29.|PDF

84. Zhao, W. #, Duan, Y. #, Li, H., Li, S., Shen, Y., Zhang, Y., Li, Y., Tang, Y. * Triazole/thiadiazole substituted 4'-demethylepipodophyllotoxin derivatives induced apoptosis in HeLa cells by up-regulating TMEM133.Eur. J. Pharmacol.2021, 905, 174189.|PDF

83. Li, C. #, Wang, X. #, Chen, X., Sheng, Q., Zhang, S., Wang, P., Quareshy M., Rihtman, B., Shao, X., Gao, C., Li, F., Li, S., Zhang, W., Zhang, X., Yang, G., Todd, J. D., Chen, Y., Zhang, Y. * A novel ATP dependent dimethylsulfoniopropionate lyase in bacteria that releases dimethyl sulfide and acryloyl-CoA.elife2021, 10, e64045.|PDF

82. Cheng, Y., Zhu, S., Guo, C., Xie, F., Jung, D., Li, S., Zhang, W., * He, S. *Microbulbifer hainanensissp. nov., a moderately halopilic bacterium isolated from mangrove sediment.Antonie van Leeuwenhoek2021, 114(7), 1033-1042.|PDF

81. Xie, B. #, Rong, J. #, Tang, B. #, Wang, S. #, Liu, G., Qin, Q., Zhang, X., Zhang, W., She, Q., Chen, Y., Li, F., Li, S., Chen, X., Luo, H. *, Zhang, Y. * Evolutionary Trajectory of the Replication Mode of BacterialReplicons.mBio2021, 12(1), e02745-20.|PDF



2020

80.  Ye, Y. #, Du, L. #, Zhang, X., Newmister, S. A., McCauley, M., Alegre-Requena, J. V., Zhang, W., Mu, S., Minami, A., Fraley, A. E., Adrover-Castellano, M. L., Carney, N. A., Shende, V. V., Qi, F., Oikawa, H., Kato, H., Tsukamoto, S., Paton, R. S., Williams, R. M.*, Sherman, D. H.*, and Li, S.* Fungal-derived Brevianamide assembly by a stereoselective semi-pinacolase. Nat. Catal. 2020,  3(6), 497-506. | PDF
 

 
79.  Li, Z., Jiang, Y., Zhang, X., Chang, Y., Li, S., Zhang, X., Zheng, S., Geng, C., Men, P., Ma, L., Yang, Y., Gao, Z., Tang, Y., and Li, S.* Fragrant venezuelaenes A and B with A5−5−6−7 tetracyclic skeleton: discovery, biosynthesis, and mechanisms of central catalysts. ACS Catal. 2020, 10, 5846-5851.  | PDF
 

78.  Li, S.*, Du, L., and Bernhardt, R.* Redox partners: Function modulators of bacterial P450 enzymes. Trends Microbiol. 2020, 28(6): 445-454. | PDF
 

 
77.  Jiang, Y., Li, Z., Zheng, S., Xu, H., Zhou, Y. J., Gao, Z., Meng, C. *, and Li, S.* Establishing an enzyme cascade for one-pot production of α-olefns from low-cost triglycerides and oils without exogenous  H2O2 addition. Biotechnol. Biofuels 2020, 13, 52. | PDF
 

76.  Li, Z. #, Jiang, Y. #, Guengerich, F. P., Ma, L., Li, S., and Zhang, W. * Engineering cytochrome P450 enzyme systems for biomedical and biotechnological applications. J. Biol. Chem. 2020, 295(3), 833-849.  | PDF
 

 
75.  Liu, K., and Li, S.* Biosynthesis of fatty acid derived hydrocarbons: perspectives on enzymology and enzyme engineering. Curr. Opin. Biotech. 2020, 62, 7-14. | PDF


 
74. Yuan, Y., He, X., Wang, T., Zhang, X., Li, Z., Xu, X., Zhang, W., Yan, X.*, Li, S.*, He S.*Efficient preparation of ba?lomycin A1 from marine Streptomyces lohii fermentation using three-phase extraction and high-speed counter-current chromatography. Mar. Drugs 2020, 18, 322. | PDF
73.  Li, F.*, Ma, L., Zhang, X., Chen, J., Qi, F., Huang, Y., Qu, Z., Yao, L., Zhang, W., Kim, E.-S.*, and Li, S.* Structure-guided manipulation of the regioselectivity of the cyclosporine A hydroxylase CYP-sb21 from Sebekia benihana. Synth. Syst. Biotechnol. 2020, 5, 236-243. | PDF
 

72.  Yang, S., Cao, X., Yu, W., Li, S., Zhou, Y. J. * Efficient targeted mutation of genomic essential genes in yeast Saccharomyces cerevisiae. Appl. Microbiol. Biotechnol. 2020, 104(7):3037-3047. | PDF
71.  Ding, L., Xu, P., Zhang, W., Yuan, Y., He, X., Su, D., Shi, Y., Naman, C. B., Yan, X., Wu, B., Lazaro, J. E. H., Li, S., and He, S.* Three new diketopiperazines from the previously uncultivable marine bacterium Gallaecimonas mangrovi HK-28 cultivated by iChip. Chem. Biodivers. 2020,17, e2000221. | PDF
70.  Fraley, A. E., Tran, H. T., Kelly, S. P., Newmister, S. A., Tripathi, A., Kato, H., Tsukamoto, S., Du, L., Li, S., Williams, R. M.*, and Sherman, D. H.* Flavin-dependent monooxygenases NotI and NotI’ mediate spiro-oxindole formation in biosynthesis of the notoamides. Chembiochem 2020, 21, 2449-2454.  | PDF
69.  Jia, K.#, Zhan, X.#, Li, H., Li, S., Shen, Y., Qi, Q., Zhang, Y., Li, Y., and Tang, Y.* A  novel  podophyllotoxin  derivative  with  higher  anti-tumor  activity produced  via  4?-demethylepipodophyllotoxin  biotransformation  by Penicillium purpurogenum. Process Biochem. 2020, 96, 220-227.  | PDF
68.  Gao, X., Zhang, X., Chen, W., Li, J., Yang, W., Zhang, X., Li, S., Liu, C.* Transcriptome analysis of Paris polyphylla var. yunnanensis illuminates the biosynthesis and accumulation of steroidal saponins in rhizomes and leaves. Phytochemistry 2020, 178, 112460.| PDF
67.  Zhao, W. #, Cong, Y. #, Li, H., Li, S., Shen, Y., Qi, Q., Zhang, Y., Li, Y., Tang, Y. * Challenges and potential for improving the druggability of podophyllotoxin-derived drugs in cancer chemotherapy. Nat. Prod. Rep. 2020,38(3), 470-488.| PDF

2019

66.  Xu, H., Liang, W., Ning, L., Jiang, Y., Yang, W., Wang, C., Qi, F., Ma, L., Du, L., Fourage, L., Zhou, Y. J. and Li, S.* Directed Evolution of P450 Fatty Acid Decarboxylases via High-Throughput Screening towards Improved Catalytic Activity. ChemCatChem 2019,12(1), 80-84. | PDF
 
 
 
65.  Zhang, W., Du, L., Qu, Z., Zhang, X., Li, F., Li, Z., Qi, F., Wang, X., Jiang, Y., Men, P., Sun, J., Cao, S., Geng, C., Qi, F., Wan, X., Liu, C., and Li, S.* Compartmentalized Biosynthesis of Mycophenolic Acid. Proc.  Natl.  Acad.  Sci.  U.S.A.  2019, 116(27), 13305-13310. | PDF
 

64.  Jiang, Y., Li, Z., Wang, C., Zhou, Y., Xu, H.*, and Li, S.* Biochemical characterization of three new α-olefn-producing P450 fatty acid decarboxylases with a halophilic property. Biotechnol. Biofuels 2019, 12, 79. | PDF
 

 
63.  Huang, Y., Zheng, X., Pilgaard, B., Holck, J., Muschiol, J., Li, S. and Lange, L. Identification and characterization of GH11 xylanase and GH43 xylosidase from the chytridiomycetous fungus, Rhizophlyctis rosea. Appl. Microbiol. Biotechnol. 2019, 103, 777-791. | PDF
62.  Jia, K., Zhu, L., Qu, X., Li, S., Shen, Y., Qi, Q., Zhang, Y., Li, Y., and Tang, Y.* Enzymatic o-glycosylation of etoposide aglycone by exploration of the substrate promiscuity for glycosyltransferases. ACS Synth. Biol. 2019, 8(12), 2718-2725. | PDF
61.  Duan, X.#, Ma, X.#, Li, S., and Zhou, Y.* Free fatty acids promote transformation eficiency of yeast. FEMS Yeast Res. 2019, 19(7), foz069. | PDF

2018

60.    Zhang, W. #, Du, L. #, Li, F., Zhang, X., Qu, Z., Han, L., Li, Z., Sun, J., Qi, F., Yao, Q., Sun, Y., Geng, C., Li, S.* Mechanistic Insights into Interactions between Bacterial Class I P450 Enzymes and Redox Partners. ACS Catal. 2018, 8, 9992-10003. | PDF
 

59.    Qi, F. #, Lei, C. #, Li, F., Zhang, X., Wang, J., Zhang, W., Fan, Z., Li, W., Tang, G., Xiao, Y.*, Zhao, G., Li, S. * Deciphering the late steps of rifamycin biosynthesis. Nat. Commun. 2018, 9, 2342. | PDF
 

58.    Chen, H., Meng, X., Xu, X., Liu, W., Li, S. * The molecular basis for lipase stereoselectivity. Appl. Microbiol. Biotechnol. 2018, 102(8), 3487-3495. | PDF
 

57.    Jiang, Y., Li, S.* The catalytic function and application of cytochrome P450 enzymes in biosynthesis and organic synthesis. Chin. J. Org. Chem. 2018, 38, 2307-2323. | PDF
56.    Zhang, X. #, Li, P. #, Qin, G., Li, S., Voogd, J. N., Tang, X.*, and Li, G.* Isolation and Absolute Con?gurations of Diversiform C17, C21 and C25 Terpenoids from the Marine Sponge Cacospongia sp. Mar. Drugs 2018, 17(1), 14. | PDF
55.    Zhong,.B., Du, L., Li, Z., Zhang, W., Zhang, X., Li, J., Huang, S., Hu, X.*, Li, S., Liu, K.* Biosynthesis of Gastrodin Based on the Cytochrome P450-Mediated Oxidative Biodegradation of 4-Cresol. J. Hunan Normal Univ. (Natural Science) 2018, 41(4), 73-80. | PDF
54.    Li, Q.*, Ding, W., Yao, Z., Tu, J., Wang, L., Huang, H., Li, S., and Ju, J.* AbmV Catalyzes Tandem Ether Installation and Hydroxylation during Neoabyssomicin/Abyssomicin Biosynthesis. Org. Lett. 2018, 20, 4854-4857. | PDF
53.    Huang, Y., Zheng, X., Pilgaard, B., Holck, J., Muschiol, J., Li, S., and Lange, L.* Identification and characterization of GH11 xylanase and GH43 xylosidase from the chytridiomycetous fungus, Rhizophlyctis rosea. Appl. Microbiol. Biotechnol. 2018, 103(2), 777-791. | PDF
52.    Dai, Z., Wang, Y., Zhou, Z., Li, S., Zhang, X *. Synthetic Biology for Production of Plant-derived Natural Products. Bull. Chin. Acad. Sci. 2018, 33(11), 1228-1238. | PDF

2017

51.    Zhang, X., and Li, S.* Expansion of chemical space for natural products by uncommon P450 reactions. Nat. Prod. Rep. 2017, 34(9), 1061-1089. | PDF
 

50.    Du, L. #, Dong, S. #, Zhang, X., Jiang, C., Chen, J., Yao, L., Wang, X., Wan, X., Liu, X., Wang, X., Huang, S., Cui, Q., Feng, Y.*, Liu, S.*, and Li, S.* Selective oxidation of aliphatic C–H bonds in alkylphenols by a chemomimetic biocatalytic system. Proc. Natl. Acad. Sci. U.S.A. 2017, 114(26), E5129-E5137. | PDF
 

49.    Li, Z., Du, L., Zhang, W., Zhang, X., Jiang, Y., Liu, K., Men, P., Xu, H., Fortman, J. L., Sherman, D. H., Yu, B., Gao, S., Li, S.* Complete elucidation of the late steps of bafilomycin biosynthesis in Streptomyces lohii. J. Biol. Chem. 2017, 292(17), 7095-7104. | PDF
 

48.    Fang, B. #, Xu, H. #, Liu, Y., Qi, F., Zhang, W., Chen, H., Wang, C., Wang, Y., Yang, W., and Li, S.* Mutagenesis and redox partners analysis of the P450 fatty acid decarboxylase OleTJE. Sci. Rep. 2017, 7, 44258. | PDF
 

47.    Xu, H., Ning, L., Yang, W., Fang, B., Wang, C., Wang, Y., Xu, J., Colin, S., Laeuffer, F., Fourage, L., and Li, S. * In vitro oxidative decarboxylation of free fatty acids to terminal alkenes by two new P450 peroxygenases. Biotechnol. Biofuels 2017, 10, 208. | PDF
46.    Sun, Y., Ma, L., Chen, H., Xu, H., Zheng, X., Qi, F., Li, S.* Production of α-alkenes catalyzed by the fused lipase and P450 fatty acid de-carboxylase. Chin. Sci. Bull. 2017, 62(1), 1-7. | PDF
 

45.    Yao, Q. #, Ma, Li. #, Liu, L., Ikeda, H., Fushinobu, S., Li, S., and Xu, L.* Hydroxylation of Compactin (ML-236B) by CYP105D7 (SAV_7469) from Streptomyces avermitilis. J. Microbiol. Biotechnol. 2017, 27(5), 956–964. | PDF
44.    Zhang, W. #, Huffman, J. #, Li, S., Shen, Y.*, and Du, L.,* Unusual acylation of chloramphenicol in Lysobacter enzymogenes, a biocontrol agent with intrinsic resistance to multiple antibiotics. BMC Biol. 2017, 17, 59-66. | PDF
43.    Zhang, W., Zhao, B., Du, L.*, Shen, Y.* Cytotoxic Polyketides with an Oxygen-Bridged Cyclooctadiene Core Skeleton from the Mangrove Endophytic Fungus Phomosis sp. A818. Molecules. 2017, 22, 1547. | PDF

2016

42.  Sun, Y. #, Ma, L. #, Han, D. #, Du, L., Qi, F., Zhang, W., Sun, J., Huang, S., Kim, E.-S.,* and Li, S.* In vitro reconstitution of the cyclosporine specific P450 hydroxylases using heterologous redox partner proteins. J. Ind. Microbiol. Biotechnol. 2016, 44(2), 161-166. | PDF
 

41.  Zhang, X., Li, Z., Du, L., Chlipala, G. E., Lopez, P. C., Zhang, W., Sherman, D. H.*, and Li, S.* Identification of an unexpected shunt pathway product provides new insights into tirandamycin biosynthesis. Tetrahedron Lett. 2016, 57(52), 5919-5923. | PDF
 

40.  Du, L., Ma, L., Qi, F., Zheng, X., Jiang, C., Li, A., Wan, X., Liu, S.-J.*, and Li, S.* Characterization of a Unique Pathway for 4-Cresol Catabolism Initiated by Phosphorylation in Corynebacterium glutamicum. J. Biol. Chem. 2016, 291(12), 6583-6594. | PDF
 

39.  Zheng, X., Fang, B., Han, D., Yang, W., Qi, F., Chen, H., and Li, S.* Improving the Secretory Expression of an α-Galactosidase from Aspergillus niger in Pichia pastoris. PLoS ONE 2016, 11(8), e0161529. | PDF
38.  Li, Z., Zhang, W., and Li, S.* Cytochrome P450 enzymes and microbial drug development – A review. Acta Microbiol. Sin. 2016, 56(3), 496-515. | PDF
37.  Sun, J., Liu, C., Li, R., Zhang, W. *, Li, S., Functional characterization of PbPT, a prenyltransferase from Penicillium Brevicompactum NRRL 864. J. Qingdao Univ. (E&T), 2016, 31(2),107-115. | PDF

2015

36.  Ma, L., Du, L., Chen, H., Sun, Y., Huang, S., Zheng, X., Kim, E.-S.*, and Li, S.* Reconstitution of the in vitro activity of the cyclosporine-specific P450 hydroxylase from Sebekia benihana and development of a heterologous whole cell biotransformation system. Appl. Environ. Microbiol. 2015, 81(18), 6268-6275. | PDF
 

35.  Yan, J.*, Liu, Y., Wang, C., Han, B., and Li, S.*Assembly of lipase and P450 fatty acid decarboxylase to constitute a novel biosynthetic pathway for production of 1-alkenes from renewable triacylglycerols and oils. Biotechnol. Biofuels 2015, 8, 34. | PDF
 

34.  Zhang, W., Cao, S., Qiu, L., Qi, F., Li, Z., Yang, Y., Huang, S., Bai, F., Liu, C., Wan, X.,* and Li, S.* Functional Characterization of MpaG’, the O-Methyltransferase Involved in the Biosynthesis of Mycophenolic Acid. Chembiochem 2015, 16(4), 565-569. | PDF
 

33.  Woo, M. W., Lee, B. R., Nah, H.J., Choi, S. S., Li, S., and Kim, E.-S.* Domain Characterization of Cyclosporin Regio-specific Hydroxylases in Rare Actinomycetes. J. Microbiol. Biotechnol. 2015, 25(10), 1634-1639. | PDF
32.  Qi, G. #, Wang, D. #, Yu, L., Tang, X., Chai, G., He, G., Ma, W., Li, S., Kong, Y., Fu, C.*, and Zhou, G.* Metabolic engineering of 2-phenylethanol pathway producing fragrance chemical and reducing lignin in Arabidopsis. Plant Cell Rep. 2015, 34(8), 1331-1342. | PDF
31.  Bernard, S. M., Akey, D. L., Tripathi, A., Park, S. R., Konwerski, J. R., Anzai, Y., Li, S., Kato, F., Sherman, D. H., and Smith, J. L.* Structural Basis of Substrate Specificity and Regiochemistry in the MycF/TylF Family of Sugar O-Methyltransferases. ACS Chem. Biol. 2015, 10(5), 1340-1351. | PDF

2014

30.  Yan, J.*, Zheng, X., Du, L., and Li, S.* Integrated lipase production and in situ biodiesel synthesis in a recombinant Pichia pastoris yeast: an efficient dual biocatalytic system composed of cell free enzymes and whole cell catalysts. Biotechnol. Biofuels 2014, 7, 55. | PDF


29.  Zhang, W., Liu, Y., Yan, J., Cao, S., Bai, F., Yang, Y., Huang, S., Yao, L., Anzai, Y., Kato, F., Podust, L. M., Sherman, D. H.*, and Li, S.* New reactions and products resulting from alternative interactions between the P450 enzyme and redox partners. J. Am. Chem. Soc. 2014, 136(9), 3640-3646. (Faculty of 1000 Prime: http://f1000.com/prime/718276150) | PDF
 

28.  Liu, Y., Wang, C., Yan, J., Zhang, W., Guan, W., Lu, X., and Li, S.* Hydrogen peroxide-independent production of α-alkenes by OleTJE P450 fatty acid decarboxylase. Biotechnol. Biofuels 2014, 7, 28. | PDF
27.  Yan, J.*, Zheng, X., and Li, S.* A novel and robust recombinant Pichia pastoris yeast whole cell biocatalyst with intracellular overexpression of a Thermomyces lanuginosus lipase: preparation, characterization and application in biodiesel production. Bioresour. Technol. 2014, 151, 43-48. | PDF
26.  Zhang, W. #, Xu, L. #, Yang, L., Huang, Y., Li, S., and Shen. Y.* Phomopsidone A, a novel depsidone metabolite from the mangrove endophytic fungus Phomopsis sp. A123. Fitoterapia 2014, 96, 146-151. | PDF
25.  Huang, S.*, Gao, J., Wu, R., Li, S. and Bai Z.* Polydimethylsiloxane: A General Matrix for High-Performance Chromatographic NMR Spectroscopy. Angew. Chem. Intl. Ed. 2014, 53, 11592-11595. | PDF
24.  Wang, Q. #, Huang, X. #, Zhang, J., Lu, X., Li, S., and Li, J.-J.* Engineering self-sufficient aldehyde deformylating oxygenase fused to alternative electron transfer systems for efficient conversion of aldehydes into alkanes. Chem. Comm. 2014, 50, 4299-4301. | PDF

2013

23.  Zhang W. #, Fortman, J. L.#, Carlson, J. C., Yan, J., Liu, Y., Bai, F., Guan, W., Jia, J., Matainaho, T., Sherman, D. H.*, and Li, S.* Characterization of the bafilomycin biosynthetic gene cluster from Streptomyces lohii. Chembiochem, 2013, 14(3), 301-306. | PDF
 

22.  Sunderhaus, J. D., McAfoos, T. J., Finefield, J. M., Kato, H., Li, S., Tsukamoto, S., Sherman, D. H., and Williams, R. M.* Synthesis and bioconversions of notoamide T: a biosynthetic precursor to stephacidin A and notoamide B. Org. Lett. 2013, 15(1), 22-25. | PDF
 

2012

21.  Li, S., Tietz, D. R., Rutaganira, F. U., Kells, P. M., Anzai, Y., Kato, F., Pochapsky, T. C., Sherman, D. H., and Podust, L. M.* Substrate recognition by the multifunctional cytochrome P450 MycG in mycinamicin hydroxylation and epoxidation reactions. J. Biol. Chem. 2012, 287(45), 37880-37890. | PDF
20.  Li, S., Anand, K., Tran, H., Yu, F., Finefield, J. M., Sunderhaus, J. D., McAfoos, T. J., Tsukamoto, S., Williams, R. M.*, and Sherman, D. H.* Comparative analysis of the biosynthetic systems for fungal bicyclo[2.2.2]diazaoctane indole alkaloids: the (+)/(-)-notoamide, paraherquamide and malbrancheamide pathways. MedChemComm 2012, 3(8), 987-996. | PDF
19.  Li, S., Finefield, J. M., Sunderhaus, J. D., McAfoos, T. J., Williams, R. M.*, and Sherman, D. H.* Biochemical characterization of NotB as an FAD-dependent oxidase in the biosynthesis of notoamide indole alkaloids. J. Am. Chem. Soc. 2012, 134(2), 788-791. | PDF
18.  Anzai, Y.*, Tsukada, S. I., Sakai, A., Masuda, R., Harada, C., Domeki, A., Li, S., Kinoshita, K., Sherman, D. H., and Kato, F. Function of the cytochrome P450 enzymes MycCI and MycG in Micromonospora griseorubida, a producer of the macrolide antibiotic mycinamicin. Antimicrob. Agents Chemother. 2012, 56(7), 3648-3656. | PDF
 

2011

17.  Carlson, J. C.#, Li, S. #, Gunatilleke, S. S., Anzai, Y., Burr, D. A., Podust, L. M., and Sherman, D. H.* Tirandamycin biosynthesis is mediated by co-dependent oxidative enzymes. Nat. Chem. 2011, 3(8), 628-633.  | PDF
16.  Akey, D. L., Li, S., Konwerski, J., Confer, L., Bernard, S. M., Anzai, Y., Kato, F., Sherman, D. H., and Smith, J. A.* New structural form in the SAM/metal-dependent O-methyltransferase family: MycE from the mycinamycin biosynthetic pathway. J. Mol. Biol. 2011, 413(2), 438-450. | PDF

2010

15.  Ding, Y., de Wet, J. R., Cavalcoli, J., Li, S., Greshock, T. J., Miller, K. A., Finefield, J. M., Sunderhaus, J. D., McAfoos, T. J., Tsukamoto, S., Williams, R. M.*, and Sherman, D. H.* Genome-based characterization of two prenylation steps in the assembly of the stephacidin and notoamide anticancer agents in a marine-derived Aspergillus sp. J. Am. Chem. Soc. 2010, 132(36), 12733-12740. | PDF
14.  McAfoos, T. J., Li, S., Tsukamoto, S., Sherman, D. H., and Williams, R. M.* Studies on the Biosynthesis of the stephacidins and notoamides. Total synthesis of notoamides. Heterocycles 2010, 82(1), 461-472. | PDF
13.  Tsukada, S., Anzai, Y., Li, S., Kinoshita, K., Sherman, D. H., and Kato, F.* Gene targeting for O-methyltransferase genes, mycE and mycF, on the chromosome of Micromonospora griseorubida producing mycinamicin with a disruption cassette containing the bacteriophage φC31 attB attachment site. FEMS Microbiol. Lett. 2010, 304(2), 148-156. | PDF
12.  Carlson, J. C., Fortman, J. L., Anzai, Y., Li, S., Douglas, A. B., and Sherman, D. H.* Identification of the tirandamycin biosynthetic gene cluster from Streptomyces sp. 307-9. Chembiochem 2010, 11(4), 564-572. | PDF
 

2009

11.  Li, S., Chaulagain, M. R., Knauff, A. R., Podust, L. M., Montgomery, J.*, and Sherman, D. H.* Selective oxidation of carbolide C-H bonds by an engineered macrolide P450 monooxygenase. Proc. Natl. Acad. Sci. U.S.A. 2009, 106(44), 18463-18468. | PDF
10.  Carlson, J. C. #, Li, S.#, Douglas, A. B., and Sherman, D. H.* Isolation and characterization of tirandamycins from a marine-derived Streptomyces sp. J. Nat. Prod. 2009, 72(11), 2076-2079. (*Co-first authors) | PDF
9.  Li, S., Anzai, Y., Kinoshita, K., Kato, F., Sherman, D. H.* Functional analysis of MycE and MycF, two O-methyltransferases involved in the biosynthesis of mycinamicin macrolide antibiotics. Chembiochem 2009, 10(8), 1297-1301. | PDF
8.  Li, S., Ouellet, H., Sherman, D. H., Podust, L. M.* Analysis of transient and catalytic desosamine-binding pockets in cytochrome P-450 PikC from Streptomyces venezuelae. J. Biol. Chem. 2009, 284(9), 5723-5730. | PDF
 

2008

7.  Anzai, Y. #, Li, S. #, Chaulagain, M. R., Kinoshita, K., Kato, F., Montgomery, J., Sherman, D. H.* Functional analysis of MycCI and MycG, cytochrome P450 enzymes involved in biosynthesis of mycinamicin macrolide antibiotics. Chem. Biol. 2008, 15(9), 950-959. | PDF

2007

6.  Li, S., Podust, L. M., Sherman, D. H.* Engineering and analysis of a self-sufficient biosynthetic cytochrome P450 PikC fused to the RhFRED reductase domain. J. Am. Chem. Soc. 2007, 129(43), 12940-12941. | PDF
5.  Li, S., Grüschow, S., Dordick, J. S., Sherman, D. H.* Molecular analysis of the role of tyrosine 224 in the active site of Streptomyces coelicolor RppA, a bacterial type III polyketide synthase. J. Biol. Chem. 2007, 282(17), 12765-12772. | PDF

 

2006

4.  Sherman, D. H.*, Li, S., Yermalitskaya, L. V., Kim, Y., Smith, J. A., Waterman, M. R., Podust, L. M.* The structural basis for substrate anchoring, active site selectivity, and product formation by P450 PikC from Streptomyces venezuelae. J. Biol. Chem. 2006, 281(36), 26289-26297. | PDF

2004

3.  Zhang, J., Zheng, Z., Huang, Y., Li, S., Su, W.* Preliminary research on antitumor activities of natural products from algal fungi. J. Xiamen Univ. (Natural Science) 2004, 43 (4), 551-556. | PDF

2003

2.    Li, S., Wang, J.*, Zheng, Z., Xu, Q., Huang, Y., Zhao, Y., Su, W. 1-(2,4-Dihydroxy-3,5-Dimethylphenyl)-ethanone (Clavatol). Acta. Cryst. 2003, E59, o1649-o1650. 
1.    Li, S., Zheng, Z.* Discovery and development of marine antitumor natural products. Mar. Sci. Bull. 2003, 22(2), 76-82. | PDF

PATENTS

1.   Li Shengying, David H. Sherman. Chimeric Cytochrome P450 Proteins and Methods of Use. (US 20090081758A3)
2.   Li Shengying, David H. Sherman, Mani Raj Chaulagain, John Montgomery, and Allison R. Knauff. A Method for Selective Oxidation of C-H Bonds by an Engineered P450 Enzyme. (WO 2011038313A2)
3.   Li Shengying, David H. Sherman, Krithika Anand, and Robert M. Williams. Biosynthetic Systems Producing Fungal Indole Alkaloids. (US 9650656 B2)
4.   Yan, Jinyong, Li Shengying. The invention relates to a method for catalyzing the production of biodiesel by coupling lipase production.（201410138507.3）
5.   Li Shengying, Liu Yi, Lv Xuefeng, and Liu Xufeng. The invention relates to a method for producing terminal olefins and its application.（201410660346.4）
6.   Yan Jinyong, Li Shengying. The invention relates to aliphatic olefin catalytic synthesis method based on coupling catalysis of lipase and P450 fatty acid decarboxylase.（ZL201510003451.5）
7.   Li Shengying, Ma Li, and Kim Eung-Soo. The invention relates to a biological transformation method for producing hair growth promoter.（201510346350.8）
8.   Li Shengying, Du lei, and Liu Shuangjiang. The invention relates to a catalytic oxidation system of phenolic compounds and its application.（201610015698.3）
9.   Li Shengying, Zheng Xianliang. The construction and application of engineering bacteria with high secretion and expression of Aspergillus Niger alpha-galactosidase AGA.（2015107066150）
10.   Li Shengying, Fang Bo, Qi Fengxia, Zhang Wei, and Du Lei. The invention relates to combination of redox partners to support the activity of P450 fatty acid decarboxylase and its application.（201610151612.X）
11.   Fourage Laurent, Laeuffer Frederie, Strub Henri, Wang Yun, Xu Jian, Xu Huifang, and Li Shengying. Production of Alpha-Olefins. (WO 2017001606A1)
12.   Li Shengying, Xu Huifang, and Ning Linlin. Method for detecting hydrogen peroxide conversion activity of an enzyme. ( EP17290115.9)
13.   Li Shengying, Xu Huifang, and Ning Linlin. Improved cytochrome P450 fatty acid decarboxylases. ( EP18290089.4)
14.   Li Shengying, Li Zhong, Du Lei, Zhang Xingwang, and Zhang Wei. The invention relates to the construction of an engineering bacteria with high yield of pavloamycin and its application.（201811634377.7）