Education & Working Experience
2023 – Now Shandong University, Associate Professor
2019 – 2023, Shandong University, Associate research professor
2013 – 2019, Chinese Academy of Sciences, Associate research professor
2009 – 2013, Chinese Academy of Sciences, Biochemistry and Molecular Biology, Ph. D
2006 – 2009, Ocean University of China, Genetics, Master
2002 – 2006, Shanxi Agricultural University, Biology Engineering, Bachelor
Research Interests
l Enzyme engineering, including: screening of industrial enzyme resources, protein evolution, P450 Enzymology and Engineering
l Biocatalysis and Biotransformation
Major Funding
l National Natural Science Foundation of China, 32071266 (国家自然科学基金，面上基金)
l The National Key Research and Development Program of China, SQ2019YFA070030 (国家重点研发计划“变革性技术关键科学问题”重点专项，子课题)
l The Fundamental Research Funds of Shandong University, 2019GN030 (山东大学基本科研业务费）
l The Applied Basic Research Programs of Science and Technology of Qingdao, 17-1-1-60-jch (青岛市应用基础研究计划项目)
l National Natural Science Foundation of China, 31600045 (国家自然科学基金，青年基金)
l Shandong Provincial Natural Science Foundation, ZR2016CQ05 (山东省自然科学基金，青年基金)
Publications
1. Qiu Z#, Liu X#, Yu J, Zhao Y, Zhao GR, Li S, Liu K*, Du L*, Ma L*. Efficient conversion of aromatic and phenylpropanoid alcohols to acids by the cascade biocatalysis of alcohol and aldehyde dehydrogenases. Synth Syst Biotechnol, 2024, 9(2):187-195.
2. Ma L, Sun T, Liu Y, Zhao Y, Liu X, Li Y, Chen X, Cao L, Kang Q, Guo J, Du L, Wang W, Li S*. Enzymatic synthesis of indigo derivatives by tuning P450 BM3 peroxygenases. Synth Syst Biotechnol, 2023, 8(3): 452-461.
3. Liu X#, Li F#, Sun T, Guo J, Zhang X, Zheng X, Du L, Zhang W, Ma L*, Li S. Three pairs of surrogate redox partners comparison for Class I cytochrome P450 enzyme activity reconstitution. Commun Biol, 2022, 5(1): 791.
4. Ma L, Li F, Zhang X, Chen H, Huang Q, Su J, Liu X, Sun T, Fang B, Liu K, Tang D, Wu D, Zhang W, Du L, Li S*. Development of MEMS directed evolution strategy for multiplied throughput and convergent evolution of cytochrome P450 enzymes. Sci China Life Sci, 2022, 65(3): 550-560.
5. Guo J, Li F, Cheng F, Ma L, Liu X, Durairaj P, Zhang G, Tang D, Long X, Zhang W, Du L, Zhang X*, Li S. Bacterial biosynthetic P450 enzyme PikCD50N: a potential biocatalyst for the preparation of human drug metabolites. J Org Chem, 2021, 86(21): 14563-14571.
6. You C, Li F, Zhang X, Ma L, Zhang YZ, Zhang W, Li S*. Structural basis for substrate specificity of the peroxisomal acyl-CoA hydrolase MpaH’ involved in mycophenolic acid biosynthesis. FEBS J, 2021, 288, 5768-5780.
7. Li F, Ma L, Zhang X, Chen J, Qi F, Huang Y, Qu Z, Yao L, Zhang W, Kim ES, Li S.Structure-guided manipulation of the regioselectivity of the cyclosporine A hydroxylase CYP-sb21 from Sebekia benihana. Synth Syst Biotechnol, 2020, 5(3): 236-243.
8. Dong S, Chen J, Zhang X, Guo F, Ma L, You C, Wang X, Zhang W, Wan X, Liu SJ, Yao LS, Li S, Du L*, Feng Y*. Structural basis for selective oxidation of phosphorylated ethylphenolsby cytochrome P450 monooxygenase CreJ. Appl Environ Microbiol, 2021, 87: e00018-21.
9. Li Z, Jiang Y, Zhang X, Chang Y, Li S, Zhang X, Zheng S, Geng C, Meng P, Ma L, Yang Y, Gao Z, Tang YJ, Li S*. Fragrant venezuelaenes A and B with a 5−5−6−7 tetracyclic skeleton: discovery, biosynthesis, and mechanisms of central catalysts. ACS catalysis, 2020, 10, 5846-5851.
10. Li Z#, Jiang Y#, Guengerich FP, Ma L, Li S, Zhang W*. Engineering cytochrome P450 enzyme systems for biomedical and biotechnological applications. J Biol Chem. 2020, 295(3): 833-849.
11. Xu H, Liang W, Ning L, Jiang Y, Yang W, Wang C, Qi F, Ma L, Du L, Fourage L, Zhou Y J, Li S*. Directed evolution of P450 fatty acid decarboxylases via high-throughput screening towards improved catalytic activity. ChemCatChem, 2019, 11, 1-6.
12. Yao Q#, Ma L#, Liu L, Ikeda H, Fushinobu S, Li S, Xu LH*. Hydroxylation of Compactin (ML-236B) by CYP105D7 (SAV_7469) from Streptomyces avermitilis. J Microbiol Biotechnol, 2017, 27(5): 956-964.
13. Sun Y#, Ma L#, Han D#, Du L, Qi F, Zhang W, Sun J, Huang S, Kim ES*, Li S*. In vitro reconstitution of the cyclosporine specific P450 hydroxylases using heterologous redox partner proteins. J Ind Microbiol Biotechnol, 2017, 44(2):161-166.
14. Du L, Ma L, Qi F, Zheng X, Jiang C, Li A, Wan X, Liu SJ*, Li S*. Characterization of a unique pathway for 4-cresol catabolism initiated by phosphorylation in Corynebacterium glutamicum. J Biol Chem, 2016, 291(12): 6583-6594.
15. Ma L, Du L, Chen H, Sun Y, Huang S, Zheng X, Kim ES, Li S*. Reconstitution of the in vitro activity of the cyclosporine-specific P450 hydroxylase from Sebekia benihana and development of a heterologous whole cell biotransformation system. Appl Environ Microbiol. 2015, 81(18), 6268-6275.
16. Li C#, Ma L#, Mou S, Wang Y, Zheng Z, Liu F, Qi X, An M, Chen H, Miao J*. Cyclobutane Pyrimidine Dimers Photolyase from Extremophilic Microalga: Remarkable UVB Resistance and Efficient DNA Damage Repair, Mutation Research-Fundamental and Molecular Mechanisms of Mutagenesis, 2015, 773: 37-42.
17. Liu X#, Song F#, Ma L, Chen C, Xiao X, Ren B, Liu X, Dai H, Piggott AM, Av-Gay Y, Zhang L*, Capon RJ*. Sydowiols A–C: Mycobacterium tuberculosis protein tyrosine phosphatase inhibitors from an East China Sea marine-derived fungus, Aspergillus sydowii. Tetrahedron Letters, 2013, 54(45): 6081-6083.
18. Ma L, Liu X, Liang H, Che Y, Chen C, Dai H, Yu K, Liu M, Ma LY, Yang CH, Song F, Wang Y*, Zhang L*. Molecular targets of 14-alpha-lipoyl andrographolide on quorum sensing in Pseudomonas aeruginosa. Antimicrob Agents Chemother, 2012, 56(12): 6088-6094.
19. Zeng X, Liu X, Bian J, Pei G, Dai H, Polyak SW, Song F, Ma L, Wang Y*, Zhang L*. Synergistic effect of AL-1 and various antibiotics on the formation of biofilms and production of exopolysaccharide and pyocyanin by Pseudomonas aeruginosa. Antimicrobial Agents and Chemotherapy, 2011, 55(6): 3015-3017.
20. Ma L, Chen J*, Liu R, Zhang XH, Jiang YA. Mutation of rpoS gene decreased resistance to environmental stresses, synthesis of extracellular products and virulence of Vibrio anguillarum. FEMS Microbiol Ecol, 2009, 70(2): 130-136.