Lei Du


State Key Laboratory of Microbial Technology, Shandong University
Tel: +86-532-58632496

Research Interests

  • Natural Product Biosynthesis, Microbial Synthetic Biology

  • Biocatalysis and Biotransformation

  • Industrial Microoganism Improvement.

Education & Working Experience

  • 2020 – Now Shandong University, Professor

  • 2018 – 2020 Chinese Academy of Sciences, Microbiology, Post Doc.

  • 2015 – 2018 Chinese Academy of Sciences, Microbiology, Ph. D

  • 2013 – 2015 Chinese Academy of Sciences, Research Assistant

  • 2009 – 2012 Tianjin University, Pharmaceutical Engineering, Master

  • 2005 – 2009 Tianjin University, Pharmaceutical Engineering, Bachelor

Major Funding

  1. The National Key Research and Development Program of China, 2021YFA0911500 (国家重点研发计划“合成生物学”重点专项,子课题)

  2. The National Key Research and Development Program of China, 2020YFA0907900 (国家重点研发计划“合成生物学”重点专项,子课题)

  3. National Natural Science Foundation of China, 32170088 (国家自然科学基金,面上基金)

  4. National Natural Science Foundation of China, 31800041 (国家自然科学基金,青年基金)

  5. Major Research Program of Shandong Provincial Natural Science Foundation, ZR2020ZD23 (山东省自然科学基金,重大基础研究项目)

  6. Postdoctoral Innovative Talents Support Program, BX20180325 (国家博士后创新人才计划项目)

  7. Shandong Provincial Natural Science Foundation, ZR2019BB024 (山东省自然科学基金)

  8. China Postdoctoral Science Foundation, 2019M652500 (国家博士后面上基金)

  9. Outstanding Postdoctoral Foundation of Chinese Academy of Sciences (中国科学院优秀博士后基金)

  10. Postdoctoral Applied Research Foundation of Qingdao (青岛应用研究项目)


  1. Dong, S.#, Chen, J.#, Zhang, X., Guo, F., Ma, L., You, C.,Wang, X., Zhang, W., Wan, X., Liu, S.-J., Yao, L.-S., Li, S., Du, L.*, and Feng, Y.* Structural basis for selective oxidation of phosphorylated ethylphenols by cytochrome P450 monooxygenase CreJ. Applied and Environmental Microbiology, 2021, 87: e00018-21. (IF: 4.792)

  2. Du, L., Li, S.* Compartmentalized biosynthesis of fungal natural products. Current Opinion in Biotechnology, 2021, 69: 128-135. (IF: 9.74)

  3. Ye, Y.#, Du, L.#, Zhang, X., Newmister, S.A., McCauley, M., Alegre-Requena, J.V., Zhang, W., Mu, S., Minami, A., Fraley, A.E., Adrover-Castellano, M.L., Carney, N.A., Shende, V.V., Qi, F., Oikawa, H., Kato, H., Tsukamoto, S., Paton, R.S., Williams, R.M.*, Sherman, D.H.*, and Li, S.*, Fungal-derived brevianamide assembly by a stereoselective semipinacolase. Nature Catalysis, 2020. 3(6): 497-506. (IF: 41.813)

  4. Du, L.#, Dong, S.#, Zhang, X., Jiang, C., Chen, J., Yao, L., Wang, X., Wan, X., Liu, X., Wang, X., Huang, S., Cui, Q., Feng, Y.*, Liu, S.-J.*, and Li, S.*, Selective oxidation of aliphatic C-H bonds in alkylphenols by a chemomimetic biocatalytic system. Proceedings of the National Academy of Sciences of the United States of America, 2017. 114: E5129-e5137. (IF: 11.205)

  5. Zhang, W.#, Du, L.#, Li, F., Zhang, X., Qu, Z., Han, L., Li, Z., Sun, J., Qi, F., Yao, Q., Sun, Y., Geng, C., and Li, S.*, Mechanistic Insights into Interactions between Bacterial Class I P450 Enzymes and Redox Partners. ACS Catalysis, 2018, 8: 9992-10003. (IF: 13.084)

  6. Du, L., Ma, L., Qi, F., Zheng, X., Jiang, C., Li, A., Wan, X., Liu, S.-J.*, and Li, S.*, Characterization of a unique pathway for 4-cresol catabolism initiated by phosphorylation in Corynebacterium glutamicum. Journal of Biological Chemistry, 2016. 291: 6583-6594. (IF: 5.157)

  7. Du, L., Liu, R.-H., Ying, L., and Zhao, G.-R.*, An efficient intergeneric conjugation of DNA from Escherichia coli to mycelia of the lincomycin-producer Streptomyces lincolnensis. International Journal of Molecular Sciences, 2012. 13: 4797-4806. (IF: 5.923)

  8. Li, Z., Li, S., Du, L., Zhang, X., Jiang, Y., Liu, W., Zhang, W., and Li, S.* Engineering bafilomycin high-producers by manipulating regulatory and biosynthetic genes in the marine bacterium Streptomyces lohii. Marine Drugs, 2021, 19: 29. (IF: 5.118)

  9. Li, S.*, Du, L., and Bernhardt, R.* Redox Partners: Function modulators of bacterial P450 enzymes. Trends in Microbiology, 2020, 28: 445-454. (IF: 17.079)

  10. Fraley, A.E., Tran, H.T., Kelly, S.P., Newmister, S.A., Tripathi, A., Kato, H., Tsukamoto, S., Du, L., Li, S., Williams, R.M.*, and Sherman, D.H.* Flavin-dependent monooxygenases NotI and NotI’ mediate spiro-oxindole formation in biosynthesis of the notoamides. ChemBioChem, 2020, 21: 2382-2382. (IF: 3.146)

  11. Zhang, W., Du, L., Qu, Z., Zhang, X., Li, F., Li, Z., Qi, F., Wang, X., Jiang, Y., Men, P., Sun, J., Cao, S., Geng, C., Qi, F., Wan, X., Liu, C., and Li, S.*, Compartmentalized biosynthesis of mycophenolic acid. Proceedings of the National Academy of Sciences of the United States of America, 2019. 116: 13305-13310. (IF: 11.205)

  12. Xu H., Liang W., Ning L., Jiang Y., Yang W., Wang C., Qi F., Ma L., Du L., Fourage L., Zhou Y.J., and Li S.* Directed evolution of P450 fatty acid decarboxylases via high-throughput screening towards improved catalytic activity. ChemCatChem, 2019, 6. (IF: 5.686)

  13. Li, Z., Du, L., Zhang, W., Zhang, X., Jiang, Y., Liu, K., Men, P., Xu, H., Fortman, J. L., Sherman, D. H., Yu, B., Gao, S., and Li, S.*, Complete elucidation of the late steps of bafilomycin biosynthesis in Streptomyces lohii. Journal of Biological Chemistry, 2017. 292: 7095-7104. (IF: 5.157)

  14. Sun, Y., Ma, L., Han, D., Du, L., Qi, F., Zhang, W., Sun, J., Huang, S., Kim, E.-S., and Li, S.*, In vitro reconstitution of the cyclosporine specific P450 hydroxylases using heterologous redox partner proteins. Journal of Industrial Microbiology and Biotechnology, 2017. 44: 161-166. (IF: 3.346)

  15. Zhang, X., Li, Z., Du, L., Chlipala, G. E., Lopez, P. C., Zhang, W., Sherman, D. H.*, and Li, S.*, Identification of an unexpected shunt pathway product provides new insights into tirandamycin biosynthesis. Tetrahedron Letters, 2016. 57: 5919-5923. (IF: 2.415)

  16. Ma, L., Du, L., Chen, H., Sun, Y., Huang, S., Zheng, X., Kim, E.-S.*, and Li, S.*, Reconstitution of the in vitro activity of the cyclosporine-specific P450 hydroxylase from Sebekia benihana and development of a heterologous whole-cell biotransformation system. Applied and Environmental Microbiology, 2015. 81: 6268-6275. (IF: 4.792)

  17. Pang, A., Du, L., Lin, C., Qiao, J., and Zhao, G.-R.*, Co-overexpression of lmbW and metK led to increased lincomycin A production and decreased byproduct lincomycin B content in an industrial strain of Streptomyces lincolnensis. Journal of Applied Microbiology, 2015, 119: 1064-1074. (IF: 3.772)

  18. Yan, J.*, Zheng, X., Du, L., and Li, S.*, Integrated lipase production and in situ biodiesel synthesis in a recombinant Pichia pastoris yeast: an efficient dual biocatalytic system composed of cell free enzymes and whole cell catalysts. Biotechnology for Biofuels, 2014, 7: 55. (IF: 6.04)