PEOPLE

Wei Zhang

Professor

Professor. Dr. Wei Zhang
Tel: +86-532-58632489
Email: zhang_wei@sdu.edu.cn

Wei Zhang, Ph.D., Professor, at Shandong University, the member of the “National outstanding youth Scholarship of NSFC”, and “Qilu Youth Scholarship of SDU”. He received his B.En. in bioengineering from Liaocheng University of China (2005). From 2005-2012, he was studied with Professor Yaojian Huang and Professor Yuemao Shen in Xiamen University for his MS and PhD studies, respectively. During 2010-2012, he spent two years as a joint Ph.D. student in the laboratory of Professor Liangcheng Du at the University of Nebraska-Lincoln, USA by the supports from “The State Scholarship Study Abroad Program for Graduate Studies at the China National University of Construction by China Scholarship Council”. In 2012, he began his academic career as an Assistant Professor in the Qingdao Institute of Bioenergy and Bioprocess Technology (QIBEBT), Chinese Academy of Sciences. In 2014, he was appointed as Associate Professor at QIBEBT. From 2016-2017, he joined the laboratory of Professor F. P. Guengerich at Vanderbilt University, TN, USA as a postdoctoral Research Associate. In 2018, he was recruited as full Professor of Shandong University.

Positions and Employment

  • 2018/07-Now, Professor, Shandong University

  • Research Fields: Biosynthetic enzymes; Natural product biosynthesis; Protein engineering.

  • 2016/10-2017/10 Postdoctoral Associate, School of Medicine, Department of Biochemistry, Vanderbilt University; Nashville, TN, USA

  • Research Fields: Biochemistry and Oxidation Mechanism of Drugs & Carcinogens Catalyzed by Human P450s.

  • 2014/01-2018/07 Associate Professor, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences;

  • Research Fields: Biosynthetic P450 enzymes; Natural product biosynthesis; Protein engineering.

  • 2012/03-2013/12 Assistant Professor, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences

  • 01/2010-05/2012 Visiting Scholar, Department of Chemistry, University of Nebraska-Lincoln; Research Fields: Natural product biosynthesis of fungal andLysobacter.

Major Funding

  1. 2021/01-2023/12 Natural Products Biosynthesis (National Natural Science Foundation of China国家自然科学基金优秀青年基金，82022066）

  2. 2020/01-2024/12 National Key R&D Program of China Grant（国家重点研发计划“合成生物学”重点专项子课题，2019YFA0905400）

  3. 2020/01-2024/12 National Key R&D Program of China Grant（国家重点研发计划“合成生物学”重点专项子课题，2019YFA0905700）

  4. 2017/08-2019/07 Reprogramming of biosynthetic pathways of high value-added natural products based on P450 catalytic parts (基于P450催化元件的高值天然产物生物合成途径重构), Major program of National Natural Science Foundation of Shandong Province (山东省自然科学基金重大基础, ZR2017ZB0207)

  5. 2016/01-2019/12 Biosynthetic mechanisms and synthetic biology of the cyclopropane unit in quinazolinone alkaloids from a marine fungus (海洋真菌喹唑啉酮生物碱中环丙烷结构单元的生物合成机制与合成生物学研究)，National Natural Science Foundation of China (国家自然科学基金面上项目，NSFC 31570030)

  6. 2014/01-2016/12 Rational modification of antibiotic structures by a cytochrome P450 monooxygenase (利用细胞色素P450 单加氧酶理性改造抗生素结构的研究), National Natural Science Foundation of China (国家自然科学基金青年基金项目，NSFC 31300075)

  7. 2016/06-2018/05 Biosynthetic pathway and heterogenerous biosynthesis of quinazolinone alkaloid cottoquinazolines (海洋真菌喹唑啉酮cottoquinazolines的异源表达)，Special grade of the financial support from the China Postdoctoral Science Foundation（中国博士后科学基金特别资助，2016M580606）

  8. 2015/01-2019/12 Youth Innovation Promotion Association of the Chinese Academy of Sciences (中国科学院青年创新促进会-青年人才计划，2015166)

  9. 2015/10-2017/09 Biosythetic mechanisms of the cyclopropane unit in quinazolinone alkaloids from a marine fungus（海洋真菌喹唑啉酮生物碱中环丙烷结构单元生物合成机制），First Grade of China Postdoctoral Science Foundation funded project（中国博士后科学基金面上项目一等资助，2015M580606）

  10. 2016/12-2018/12 Development of high-yield cell factory of drug-lead natural product Bafilomycin A1 (生物医药先导化合物巴弗洛霉素A1高产细胞工厂构建的关键技术研究及应用), Primary Research & Development Plan of Shandong Province（山东省重点研发计划，2016GSF121001）

  11. 2015/09-2017/08 Rational design and modification of antibiotics by a cytochrome P450 monooxygenase (利用P450理性设计与改造抗生素的研究),（青岛市应用基础研究计划-青年专项）

  12. 2015/04-2017/03 The biological modification and potential application of bafilomycin from marineStreptomyces lohii(海洋链霉菌天然产物巴弗洛霉素的改造及应用), (青岛市博士后人员应用研究, 861605040068)

Publications

  1. Zhong Li(#), Yuanyuan Jiang(#), Li Ma, F. Peter Guengerich, Shengying Li,Wei Zhang*. Engineering cytochrome P450 enzyme systems for practical catalysis.J Biol Chem,220, 295: 833-849. (Nature Index).

  2. Wei Zhang, Lei Du, Zepeng Qu,Xingwang Zhang, Fengwei Li,Zhong Li, Feifei Qi, Xiao Wang, Yunayuan Jiang, Ping Men, Jingran Sun, Shaona Cao, Ce Geng, Fengxia Qi, Xiaobo Wan, Changning Liu, Shengying Li*. Compartmentalized biosynthesis of mycophenolic acid.Proc. Acad. Natl. Sci. U.S.A., 2019, 116: 13305-13310. (Nature Index).

  3. Wei Zhang(#), Lei Du(#), Fengwei Li, Xingwang Zhang, Zepeng Qu, Lei Han, Zhong Li, Jingran Sun, Fengxia Qi, Qiuping Yao, Yue Sun, Ce Geng, Shengying Li*. Mechanistic insights into interactions between bacterial class I P450 enzymes and redox partners,ACS Catal., 2018, 8: 9992−10003 (Cover Paper) (5-Year IF: 11.783 )

  4. Wei Zhang, Yi Liu, Jinyong Yan, Shaona Cao, Fali Bai, Ying Yang, Shaohua Huang, Lishan Yao, Yojiro Anzai, Fumio Kato, Larissa M. Podust, David H. Sherman*, and Shengying Li*, New reactions and products resulting from alternative interactions between the P450 enzyme and redox partners.J. Am. Chem. Soc., 2014, 136 (9), 3640−3646. (F1000,Nature Index).

  5. Wei Zhang，Shaona Cao， Li Qiu， Fengxia Qi， Zhong Li， Ying Yang， Shaohua Huang， Fali Bai， Changning Liu， Xiaobo Wan *， Shengying Li *，Functional characterization of MpaG', the O-Methyltransferase involved in the biosynthesis of mycophenolic acid，ChemBioChem, 2015, 16: 565-569.

  6. Wei Zhang(#), J. L. Fortman(#), Jacob C. Carlson, Jinyong Yan, Yi Liu, Fali Bai, Wenna Guan, Junyong Jia, Teatulohi Matainaho, David H. Sherman*, and Shengying Li*. Characterization of the bafilomycin biosynthetic gene cluster fromStreptomyces lohii.ChemBioChem, 14 (3): 301-306, 2013.

  7. Wei Zhang(#), Liyan Xu(#), Lishan Yang, Yaojian Huang , Shengying Li , Yuemao Shen*, Phomopsidone A, a novel depsidone metabolite from the mangrove endophytic fungusPhomopsissp. A123.Fitoterapia,2014, 96, 146–151.

  8. Wei Zhang(#), Yaoyao Li(#), Guoliang Qian, Yan Wang, Haotong Chen, Yuezhong Li, Fengquan Liu, Yuemao Shen*, and Liangcheng Du*. Identification and Characterization of the Anti-Methicillin-ResistantStaphylococcus aureusWAP-8294A2 Biosynthetic Gene Cluster fromLysobacter enzymogenesOH11.Antimicrob. Agents Chemother. 55 (12): 5581-5589, 2011.

  9. Wei Zhang(#), Mingzi Wang(#), Yaojian Huang, Sampson. K. P. Chea, Zhonghui Zheng, Xiaoming Qian*, and Yuemao Shen. New and highly efficient methodology for screening high-yield strains of cytotoxic deacetylmycoepoxydiene (DAM).Lett. Appl. Microbiol.52 (5): 441-447, 2011.

  10. Wei Zhang(#), Justin Huffman(#), Shengying Li, Yuemao Shen*, Liangcheng Du*, Unusual acylation of chloramphenicol inLysobacter enzymogenes, a biocontrol agent with intrinsic resistance to multiple antibiotics,BMC Biotechnology, 2017; 17: 59. doi: 10.1186/s12896-017-0377-y.

  11. Jingran Sun, Changning Liu, Ronggui Li,Wei Zhang*, Shengying Li, Functional characterization of PbPT, a prenyltransferase fromPenicillium brevicompactumNRRL 864.Journal of Qingdao University(Engineering & Technology Edition), 2016, 31(2):107-115. （孙靖然； 刘长宁； 李荣贵；张伟*； 李盛英；短密青霉NRRL 864异戊烯基转移酶PbPT的克隆表达及功能分析，青岛大学学报(工程技术版)，2016, 31(2):107-115.）

  12. Zhong Li,Wei Zhang, Shengying Li *, Cytochrome P450 enzymes and microbial drug development – A review.Acta Microbiologica Sinica2016, 56(3), 496-515. （李众，张伟, 李盛英*; 细胞色素P450酶与微生物药物创制，微生物学报,56（03）：496~515, 2015.）

  13. Yi Liu, Cong Wang, Jinyong Yan,Wei Zhang, Wenna Guan, Xuefeng Lu and Shengying Li.* Hydrogen peroxide-independent production ofα-alkenes by OleTJE P450 fatty acid decarboxylase.Biotechnol. Biofuels,7: 28-40, 2014.

  14. Chunxue Wang, Pradeep S. Pallan,Wei Zhang, Li Lei, Francis K. Yoshimoto, Michael R. Waterman, Martin Egli and F. Peter Guengerich*，Functional analysis of human cytochrome P450 21A2 variants involved in congenital adrenal hyperplasia.J. Biol. Chem.,2017, 292(26): 10767–10778. doi: 10.1074/jbc.M117.792465.

  15. Zhong Li, Lei Du,Wei Zhang, Xingwang Zhang，Yuanyuan Jiang，Kun Liu, Ping Men，Huifang Xu，Jeffrey L. Fortman，David H. Sherman，Bing Yu， Xingwang Zhang，Song Gao，Shengying Li *, Complete elucidation of the late steps of bafilomycin biosynthesis inStreptomyces lohii.J. Biol. Chem.,2017, 292(17): 7095-7104.

  16. Bo Fang, Huifang Xu, Yi Liu, Fengxia Qi,Wei Zhang, Cong Wang, Yilin Wang, Wenxia Yang, Shengying Li*, Mutagenesis and redox partners analysis of the P450 fatty acid decarboxylase OleTJE.Sci. Rep.2017, 7, 44258; DOI: 10.1038/srep44258.

  17. Yue Sun, Li Ma, Dongfei Han, Lei Du, Feifei Qi,Wei Zhang, Jingran Sun, Shaohua Huang, E.-S Kim,* and Shengying Li*,In vitroreconstitution of the cyclosporine specific P450 hydroxylases using heterologous redox partner proteins.J. Ind. Microbiol. Biotechno,2017, 44(2): 161–166,.

  18. Xingwang Zhang, Zhong Li, Lei Du, George E. Chlipala, Patricia C. Lopez,Wei Zhang, David H. Sherman*, Shengying Li*, Identification of an unexpected shunt pathway product provides new insights into tirandamycin biosynthesis,Tetrahedron Letters, 2016, 57(52): 5919-5923.

  19. Mingzi Wang,Wei Zhang, Weikai Xu, Yuemao Shen *, and Liangcheng Du*, Optimization of genome shuffling for high yield production of the antitumor deacetylmycoepoxydiene in an endophytic fungus of mangrove plants.Applied Microbiology and Biotechnology, 2016, 100(17):7491-7498.

  20. Jifeng Wang, Baobing Zhao, Yuting Yi,Wei Zhang, Xuan Wu, Lianru Zhang, Yuemao Shen*. Mycoepoxydiene, a fungal polyketide inhibits MCF-7 cells through simultaneously targeting p53 and NF-κB pathways.Biochem. Pharm., 84(7), 891-899, 2012.

  21. Qiang Chen, Tenghui Chen, Yaojian Huang, Wenjiao Li, Yang Li, Yuemao Shen,Wei Zhang, Jingwei Zhu, Chundong Yu*. Mycoepoxydiene Inhibits Lipopolysaccharide-Induced Inflammatory Responses through the of TRAF6 Polyubiquitination.PloS one.7 (9): e44890, 2012.

  22. Yunxuan Xie,Wei Zhang, Yaoyao Li, Mingzi Wang, Ronald L. Cern, Yuemao Shen and Liangcheng Du*. Transformation ofFusarium verticillioideswith a polyketide gene cluster isolated from a fungal endophyte activates the biosynthesis of fusaric acid.Mycology2 (1): 24, 2011.

  23. Jifeng Wang, Baobing Zhao,Wei Zhang, Xuan Wu, Ruoyu Wang, Yaojian Huang, Dong Chen, Kum Park, Bart C. Weimer*, Yuemao Shen*. Mycoepoxydiene, a fungal polyketide, induces cell cycle arrest at the G2/M phase and apoptosis in HeLa cells.Bioorg. Med. Chem. Lett. 20, 7054, 2010.

Major Awards

  • 2020 The Recruitment member of National outstanding youth fund winner，NSFC

  • 2016 Special grade of the financial support from the China Postdoctoral Science Foundation

  • 2015 1st Class funding of China Postdoctoral Science Foundation funded project

  • 2015 The Recruitment member of Youth Innovation Promotion Association，CAS

  • 2010 The State Scholarship Study Abroad Program by China Scholarship Council